Question Detail

MIC 121 Microscopes Objective Question

Question

What is a primary limitation of the Bright-field microscope when observing living cells?
Options
A It can only be used with stained specimens.
B The specimen appears dark against a bright background, making contrast difficult.
C Absence of contrast between the specimen and the surrounding medium.
Correct Answer
D It uses ultraviolet light which is harmful to living cells.
Correct Answer

Option C is the correct answer.

Detailed Explanation

Page 5 identifies a limitation of the Bright-field microscope as: 'Absence of contrast between the specimen and the surrounding medium, which makes it difficult to observe living cells'. This often necessitates stained specimens.

Hint

Consider why staining is often required for bright-field microscopy.

Question Info