Question Detail
Question
What is a primary limitation of the Bright-field microscope when observing living cells?
Correct Answer
Option C is the correct answer.
Detailed Explanation
Page 5 identifies a limitation of the Bright-field microscope as: 'Absence of contrast between the specimen and the surrounding medium, which makes it difficult to observe living cells'. This often necessitates stained specimens.
Hint
Consider why staining is often required for bright-field microscopy.