Question Detail

MIC 121 Microscopes Objective Question
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Question

In Fluorescence microscopy, what is the role of the fluorochrome, and how does it relate to the emitted light?
Options
A The fluorochrome absorbs longer wavelength light and releases shorter wavelength light.
B The fluorochrome stains the background dark, while the specimen remains bright.
C The fluorochrome is absorbed by the specimen and then releases fluorescent light of a longer wavelength.
Correct Answer
D The fluorochrome blocks all light except what is reflected by the specimen.
Correct Answer

Option C is the correct answer.

Detailed Explanation

Page 10 describes the process: 'The light is absorbed by the specimen stained with fluorochrome and releases fluorescent (or green) light of a longer wavelength'.

Hint

Fluorescence is about absorbing high-energy light and re-emitting lower-energy light.

Question Info

Course: MIC 121
Topic: Microscopes
Type: Objective
Difficulty: Not specified
Question 24 of 990
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