Question Detail

MIC 121 Microscopes Objective Question

Question

What is the primary limitation of a bright-field microscope when attempting to observe living cells?
Options
A It can only be used in dark environments.
B It requires electron beams which can harm living cells.
C Absence of contrast between the specimen and the surrounding medium.
Correct Answer
D The tungsten light beam generates too much heat.
Correct Answer

Option C is the correct answer.

Detailed Explanation

Page 5 explicitly states the 'Limitation: Absence of contrast between the specimen and the surrounding medium, which makes it difficult to observe living cells'.

Hint

Consider why living, unstained cells might be hard to see clearly against their background under this type of microscope.

Question Info