Question Detail
Question
What is a primary limitation of the bright-field microscope when observing living cells?
Correct Answer
Option C is the correct answer.
Detailed Explanation
According to page 5, a primary limitation of the bright-field microscope is the 'Absence of contrast between the specimen and the surrounding medium, which makes it difficult to observe living cells'. This often necessitates staining nonviable cells to increase contrast.
Hint
Think about what makes it hard to see unstained, live microbes clearly against their surroundings.