Question Detail

MIC 121 Microscopes Objective Question
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Question

How does a dark-field microscope create its characteristic image where the specimen appears bright against a dark background?
Options
A By staining the specimen with a fluorescent dye
B By using electron beams instead of light rays
C By blocking direct light and only allowing reflected or refracted light to enter the objective lens
Correct Answer
D By using two sets of lenses for high magnification
Correct Answer

Option C is the correct answer.

Detailed Explanation

Page 7 describes the mechanism: 'An opaque disk of this condenser blocks the light that would enter the objective lens directly. The only light that is reflected or refracted by the specimens enters the objective lens and forms an image. The field surrounding a specimen appears dark and the specimen appears brightly illuminated'.

Hint

Think about what the opaque disk in the dark-field condenser does to the illumination path.

Question Info

Course: MIC 121
Topic: Microscopes
Type: Objective
Difficulty: Not specified
Question 48 of 990
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