Question Detail

MIC 121 Microscopes Objective Question

Question

After a specimen stained with a fluorochrome is exposed to shorter wavelength light, what is the resulting characteristic of the image produced by a fluorescence microscope?
Options
A A dark specimen against a bright background
B A magnified but unstained image
C A bright image on a dark background
Correct Answer
D A distorted image due to staining
Correct Answer

Option C is the correct answer.

Detailed Explanation

Page 10 states: 'The light is absorbed by the specimen stained with fluorochrome and releases fluorescent (or green) light of a longer wavelength. This produces a bright image on a dark background.'

Hint

The phenomenon of fluorescence means the specimen emits light, which is then observed against a dark field.

Question Info