Question Detail

MIC 121 Microscopes Objective Question

Question

What is the primary limitation of a Bright-field microscope when observing living cells?
Options
A It has very low magnification capabilities.
B It requires electron beams, which can damage living cells.
C There is an absence of contrast between the specimen and the surrounding medium.
Correct Answer
D It can only be used with stained, nonviable specimens.
Correct Answer

Option C is the correct answer.

Detailed Explanation

The text states, 'Limitation: Absence of contrast between the specimen and the surrounding medium, which makes it difficult to observe living cells.' This is the key challenge for bright-field microscopy with live, unstained samples.

Hint

Think about what makes it hard to see a transparent object against a similar background.

Question Info