Question Detail
Question
What is the primary limitation of a Bright-field microscope when observing living cells?
Correct Answer
Option C is the correct answer.
Detailed Explanation
The text states, 'Limitation: Absence of contrast between the specimen and the surrounding medium, which makes it difficult to observe living cells.' This is the key challenge for bright-field microscopy with live, unstained samples.
Hint
Think about what makes it hard to see a transparent object against a similar background.