Question Detail

MIC 121 Microscopes Objective Question
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Question

In Fluorescence Microscopy, what is the role of a 'fluorochrome'?
Options
A It is a type of lens used to magnify the image.
B It is a fluorescent dye that stains the specimen and then releases light of a longer wavelength after absorbing shorter wavelength light.
Correct Answer
C It is a filter that blocks unwanted light from reaching the objective.
D It is a part of the condenser that focuses the light beam.
Correct Answer

Option B is the correct answer.

Detailed Explanation

The text states, 'A specimen is stained with a fluorescent dye (fluorochrome) and then exposed to light of a shorter wavelength... The light is absorbed by the specimen stained with fluorochrome and releases fluorescent (or green) light of a longer wavelength'.

Hint

Think about the meaning of 'fluorescence' and how a dye contributes to it.

Question Info

Course: MIC 121
Topic: Microscopes
Type: Objective
Difficulty: Not specified
Question 68 of 990
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