Question Detail

CHM 102 Practice Questions Objective Question

Question

Which of the following is the first step in the determination of the primary structure of proteins?
Options
A determining the number and kind of amino acids in the peptide
B reducing the disulfide bridges in the protein
Correct Answer
C protecting the N-terminal of the peptide
D protecting the C-terminal of the peptide
Correct Answer

Option B is the correct answer.

Detailed Explanation

The first step in determining the primary structure of a protein is to reduce any disulfide bridges (S-S bonds) to prevent cross-linking and allow the peptide chains to be separated.

Hint

What must be done to disulfide bonds first?

Question Info