Question Detail

MIC 121 MIC 121 Test compilation 3 Objective Question

Question

What is a characteristic of negative staining?
Options
A It requires heat-fixing the bacterial smear
B It does not require making a smear and stains the background
Correct Answer
C It uses two different stains to differentiate bacteria
D It uses basic dyes such as methylene blue or basic fuchsin
Correct Answer

Option B is the correct answer.

Detailed Explanation

Negative staining involves staining the background surrounding the specimen, leaving the cells themselves unstained. This method does not typically require heat-fixing, which can distort cell morphology, making it useful for observing natural cell size and shape, and often capsules.

Hint

Think about what 'negative' means in this context – it's about what is *not* stained.

Question Info