Question Detail

MIC 121 Laboratory safety and ethics in microbiology Objective Question

Question

What is the 'gold standard' sterilisation method that effectively destroys all life, including endospores, but is not reliably effective against prions?
Options
A Dry Heat (160°C / 2 h)
B 70% Ethanol
C Autoclaving (121°C, 15 psi, 15-30 min)
Correct Answer
D UV Irradiation
Correct Answer

Option C is the correct answer.

Detailed Explanation

Page 18, under 'Autoclaving,' states: 'Gold standard. Destroys all life, including endospores. ...Note: Prions are NOT reliably destroyed by standard autoclaving.'

Hint

This method uses moist heat and pressure.

Question Info