Question Detail

MIC 121 LaboratorySafety andEthicsin Microbiology Objective Question
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Question

Which of the following practices is considered essential for maintaining aseptic technique when working with microbial cultures?
Options
A Resting stoppers or caps face-down on the bench surface to prevent contamination from above.
B Cooling the inoculation loop until it is completely cold before contacting cultures.
C Manipulating cultures within 15–20 cm of a Bunsen burner.
Correct Answer
D Keeping vessels open for extended periods to allow air circulation.
Correct Answer

Option C is the correct answer.

Detailed Explanation

Page 6, 'Aseptic Technique', point 2 states: 'Manipulate cultures within 15–20 cm of a Bunsen burner where convection currents exclude airborne contaminants.' Resting lids down (point 4) is a contamination risk, cooling the loop (point 5) should be for 'several seconds' not 'completely cold' to avoid heat-killing organisms, and minimizing exposure (point 3) means keeping vessels open for the 'shortest possible time'.

Hint

Consider the role of the Bunsen burner in creating a sterile working environment.

Question Info

Course: MIC 121
Topic: LaboratorySafety andEthicsin Microbiology
Type: Objective
Difficulty: Not specified
Question 673 of 984
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