Question Detail

MIC 121 LaboratorySafety andEthicsin Microbiology Objective Question

Question

Which sterilization method is described as the 'gold standard' and is effective in destroying all life, including endospores?
Options
A Dry Heat (160°C / 2 h)
B Membrane Filtration (0.22 µm)
C Autoclaving (121°C, 15 psi, 15-30 min)
Correct Answer
D 70% Ethanol
Correct Answer

Option C is the correct answer.

Detailed Explanation

Page 18, 'Sterilisation and Disinfection Methods', describes 'Autoclaving (121°C, 15 psi, 15-30 min)' as the 'Gold standard. Destroys all life, including endospores.'

Hint

This method uses moist heat and pressure to achieve high levels of sterilization.

Question Info