Question Detail

MIC 121 LaboratorySafety andEthicsin Microbiology Objective Question

Question

Which sterilization method is considered the 'gold standard' and effectively destroys all life, including endospores, but is not reliably effective against prions?
Options
A Dry Heat
B 70% Ethanol
C Autoclaving
Correct Answer
D Membrane Filtration
Correct Answer

Option C is the correct answer.

Detailed Explanation

The document describes 'Autoclaving (121°C, 15 psi, 15-30 min)' as the 'Gold standard. Destroys all life, including endospores. Verify with spore strips + chemical indicator tape. Note: Prions are NOT reliably destroyed by standard autoclaving.'

Hint

Look for the method explicitly labeled as the 'Gold standard' in the 'Sterilisation and Disinfection Methods' section.

Question Info