Question Detail

MIC 121 Mcb compilations Objective Question

Question

Which of the following is true about simple staining?
Options
A It uses dyes like India ink or Nigrosin
B It requires two different dyes
C It produces color contrast and imparts the same color to all bacteria in the smear
Correct Answer
D It does not require making a smear
Correct Answer

Option C is the correct answer.

Detailed Explanation

Simple staining involves using a single basic dye to stain all cells in a smear, producing a uniform color contrast against the unstained background. India ink and Nigrosin are used in negative staining, which stains the background, not the cells. Differential staining (like Gram stain) requires two or more dyes.

Hint

Consider the primary goal of simple staining in microscopy, which is to make cells visible under the microscope by coloring them uniformly.

Question Info