Question Detail
Question
What is the principle behind the functioning of an autoclave for sterilization?
Correct Answer
Option A is the correct answer.
Detailed Explanation
An autoclave utilizes moist heat (steam under pressure) to achieve sterilization. The combination of high temperature and high pressure steam is highly effective at denaturing proteins and destroying microbial cells, including endospores.
Hint
Autoclaves are essentially high-pressure steam sterilizers. What kind of heat does steam represent?