Question Detail

MIC 121 Mcb compilations Objective Question

Question

Which of the following is true about simple staining?
Options
A It uses dyes like India ink or Nigrosin
B It requires two different dyes
C It produces color contrast and imparts the same color to all bacteria in the smear
Correct Answer
D It does not require making a smear
Correct Answer

Option C is the correct answer.

Detailed Explanation

Simple staining uses a single basic dye (e.g., methylene blue, crystal violet) to color all bacterial cells uniformly. This increases the contrast between the cells and the background, making their morphology and arrangement easily observable. Options A and B describe negative staining and differential staining, respectively. Option D is incorrect, as a smear is typically required.

Hint

Think about the primary goal of using a single dye to stain bacteria.

Question Info