Question Detail
Question
What is the principle behind the functioning of an autoclave for sterilization?
Correct Answer
Option A is the correct answer.
Detailed Explanation
An autoclave utilizes moist heat, in the form of steam under pressure, as its principle for sterilization. The high pressure allows the steam to reach temperatures above the boiling point of water (typically 121°C or 132°C), which is highly effective at denaturing proteins, melting lipids, and destroying all forms of microbial life, including spores.
Hint
Autoclaves use steam at high temperatures and pressures. What type of heat is steam?