Question Detail

MIC 121 Mib compilations3 Objective Question

Question

Which of the following best describes Phase Contrast microscopy?
Options
A It distinguishes between solid and liquid phases of the cell.
B It changes the color of light passing through it.
C It continuously changes the phase of the incident light from the condenser to improve contrast in the specimen.
D It transforms subtle changes in light waves passing through the cell to contrast parts of the cell with differences in light intensity.
Correct Answer
Correct Answer

Option D is the correct answer.

Detailed Explanation

Phase-contrast microscopy is a powerful technique for viewing unstained, live specimens. It works by converting slight variations in the refractive index (and thus phase shifts) of light as it passes through different parts of the specimen into measurable differences in light intensity (brightness or darkness), thereby enhancing contrast.

Hint

The name 'phase contrast' directly indicates the optical property it manipulates to create an image.

Question Info