Question Detail

BIO 201 Objective Question

Question

In a sandwich ELISA diagnostic assay, what structural feature must the target antigen possess?
Options
A At least two distinct spatial epitopes for antibody binding
Correct Answer
B A single catalytic active site for substrate cleavage
C A hydrophobic transmembrane domain
D Covalent attachment to fluorescent dye molecules
Correct Answer

Option A is the correct answer.

Detailed Explanation

Sandwich ELISAs require the target analyte to be bound simultaneously by a capture antibody and a detection antibody at distinct non-overlapping epitopes.

Hint

No hint available

Question Info