Question Detail

BIO 201 Objective Question

In parallel quantitative Real-Time PCR (qPCR) assays, what is the role of running a housekeeping gene in parallel with the target gene?

A To normalize for differences in total RNA input and extraction efficiency across samples.

Correct Answer

B To amplify the target fluorescence signal past the quantification threshold.

C To suppress non-specific primer dimer formation in the target master mix.

D To increase the optical absorbance maximum of the fluorescent dye.

Option A is the correct answer.

Parallel reaction wells containing housekeeping genes control for variability in nucleic acid loading and enzymatic efficiency.

No hint available

Course: BIO 201

Type: Objective

Difficulty: Not specified

Question 69 of 285

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