Question Detail

BIO 201 Objective Question

Question

When constructing a parallel standard calibration curve in diagnostic assays, what function does a zero-calibrator (blank) serve?
Options
A To measure baseline background signal contributed by reagents and matrix in the absence of target analyte.
Correct Answer
B To set the optical saturation limit for the photodiode reader.
C To calculate the maximum binding capacity ($B_{\max}$) of standard microplates.
D To stabilize the pH of the wash buffer solution.
Correct Answer

Option A is the correct answer.

Detailed Explanation

The blank controls for non-specific background noise, allowing subtraction of baseline interference from analytical measurements.

Hint

No hint available

Question Info