Question Detail

BIO 201 Objective Question

Question

What primary operational parameter must be strictly standardized across parallel lanes in gel electrophoresis diagnostics?
Options
A Voltage gradient, temperature, and gel matrix composition.
Correct Answer
B The physical volume of DNA ladder loaded into test sample wells.
C The ambient light levels present in the laboratory room during the run.
D The length of time samples are incubated at 95 °C prior to loading.
Correct Answer

Option A is the correct answer.

Detailed Explanation

Uniform voltage, temperature, and buffer/gel conditions ensure that electrophoretic mobility depends strictly on molecule size/charge, not lane position.

Hint

No hint available

Question Info