Question Detail

BIO 201 Objective Question
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Question

What is the primary function of blocking buffers (e.g., BSA or non-fat milk) in parallel Western blot or ELISA assays?
Options
A To saturate unoccupied hydrophobic binding sites on membranes or plate wells, preventing non-specific antibody binding.
Correct Answer
B To cleave target protein substrates into smaller antigenic peptide fragments.
C To act as a enzymatic cofactor for horseradish peroxidase substrate turnover.
D To maintain optimal sample viscosity during automated microplate pipetting.
Correct Answer

Option A is the correct answer.

Detailed Explanation

Blocking agents cover open binding surfaces so detection antibodies bind exclusively to target antigens, reducing background noise.

Hint

No hint available

Question Info

Course: BIO 201
Type: Objective
Difficulty: Not specified
Question 124 of 285
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