Question Detail

BIO 201 Objective Question
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Question

During parallel execution of diagnostic qPCR, what does a high $C_q$ (quantification cycle) value signify?
Options
A A low initial concentration of target nucleic acid template in the sample.
Correct Answer
B A high initial concentration of target nucleic acid template in the sample.
C Superior enzymatic activity of the Taq DNA polymerase enzyme.
D Severe contamination of negative template control (NTC) wells.
Correct Answer

Option A is the correct answer.

Detailed Explanation

$C_q$ is inversely proportional to target copy number; more amplification cycles are required to cross the threshold when starting template amount is low.

Hint

No hint available

Question Info

Course: BIO 201
Type: Objective
Difficulty: Not specified
Question 130 of 285
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