Question Detail

BIO 101 BIO 101 Past Questions Objective Question
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Question

Why is a 'blank' cuvette used before measuring experimental samples in a spectrophotometer?
Options
A To calibrate absorbance to 1.000
B To calibrate baseline absorbance of the solvent to zero
Correct Answer
C To rinse the internal light diffraction grating
D To test if the photodetector bulb is warm
Correct Answer

Option B is the correct answer.

Detailed Explanation

A blank containing solvent alone subtracts background absorbance, ensuring measurements reflect only the solute.

Hint

No hint available

Question Info

Course: BIO 101
Topic: BIO 101 Past Questions
Type: Objective
Difficulty: Not specified
Question 2424 of 2556
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