Question Detail
Question
What causes the mitochondrial matrix to be negatively charged compared to the intermembrane space?
Correct Answer
Option B is the correct answer.
Detailed Explanation
The active pumping of positively charged protons (H+) out of the matrix leaves behind an excess of negative charges (like OH- ions), making the matrix electrically negative relative to the intermembrane space. This creates both a chemical concentration gradient and an electrical potential.
Hint
Removing positive charges makes a space more negative.