Question Detail
Question
How do uncoupling agents affect oxidative phosphorylation?
Correct Answer
Option A is the correct answer.
Detailed Explanation
Uncouplers (like DNP) are lipid-soluble molecules that shuttle protons across the inner mitochondrial membrane back into the matrix without going through ATP synthase. This dissipates the proton gradient, so electron transport continues (and even increases, consuming oxygen and fuel) but ATP synthesis is uncoupled from it.
Hint
They 'short-circuit' the proton gradient, releasing energy as heat.