Question Detail

ZOO 101 ZOO 101 Past Questions Objective Question

Question

In forensic study, DNA fingerprinting processes used to determine whether or not an individual is the parent of a child by
Options
A VNTR loci analysis
Correct Answer
B RFLP analysis
C Degenerate oligonucleotides assay
D c-DNA analysis
Correct Answer

Option A is the correct answer.

Detailed Explanation

VNTR (Variable Number Tandem Repeat) loci analysis is a DNA fingerprinting technique used in parentage determination. It analyzes regions of the genome where short DNA sequences are repeated in tandem, and the number of repeats varies among individuals. RFLP (Restriction Fragment Length Polymorphism) is an earlier method also used for fingerprinting. For parentage testing, analysis of highly polymorphic VNTR or STR (Short Tandem Repeat) loci is standard. Degenerate oligonucleotides are used in PCR for related sequences; cDNA analysis is for expressed genes.

Hint

This technique analyzes highly variable repetitive DNA regions that are inherited in a Mendelian fashion.

Question Info