Question Detail

ZOO 101 ZOO 101 Past Questions Objective Question

Question

Assertion (A): Using recombinant DNA technology, desired genes can be implanted into a bacterium for amplification and expression. Reason (R): Any bacterium can carry expression vector.
Options
A Both A and R are individually true and R is the correct explanation of A
B Both A and R are individually true but R is not the correct explanation of A
C A is true but R is false
Correct Answer
D A is false but R is true
Correct Answer

Option C is the correct answer.

Detailed Explanation

Assertion A is true: Recombinant DNA technology allows insertion of desired genes into vectors (e.g., plasmids) that can be introduced into bacteria (such as E. coli) for amplification (cloning) and expression of the gene product. Reason R is false: Not any bacterium can carry an expression vector. Specific bacterial strains (e.g., competent E. coli) with appropriate genetic modifications are used, and vectors have specific host ranges (e.g., Gram-negative vectors do not work in Gram-positive bacteria). The statement is an overgeneralization.

Hint

Consider the host specificity of vectors and the requirements for successful transformation and expression.

Question Info