Question Detail

MIC 121 LaboratorySafety andEthicsin Microbiology Objective Question
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Question

HEPA filtration is crucial for removing particles in Biosafety Cabinets. What is the efficiency stated for HEPA filters, and what is a key limitation of UV-C irradiation as a supplementary disinfection method?
Options
A Removes ≥99.97% of particles ≥0.3 µm; UV-C is effective only on living cells.
B Removes ≥99.97% of particles ≥0.3 µm; UV-C is only supplementary and line-of-sight only.
Correct Answer
C Removes ≥95% of particles ≥0.5 µm; UV-C is corrosive to equipment.
D Removes all particles; UV-C damages all nucleic acids indiscriminately.
Correct Answer

Option B is the correct answer.

Detailed Explanation

The 'Sterilisation and Disinfection Methods' section states: 'HEPA removes ≥99.97% of particles ≥0.3 µm; used in BSCs. UV-C (254 nm) damages nucleic acids — supplementary only, line-of-sight only.'

Hint

Recall the specific percentage and particle size for HEPA, and the spatial constraint for UV-C.

Question Info

Course: MIC 121
Topic: LaboratorySafety andEthicsin Microbiology
Type: Objective
Difficulty: Not specified
Question 730 of 984
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