Question Detail
Question
During lyophilisation of liquid culture of a microorganism, it is frozen with dry ice and exposed to vacuum for _______ to remove all the water.
Correct Answer
Option C is the correct answer.
Detailed Explanation
Lyophilisation, or freeze-drying, involves freezing the sample and then applying a vacuum to cause the frozen water to directly transition from solid to gas (sublimate), bypassing the liquid phase.
Hint
This process is also known as freeze-drying. What physical process allows ice to turn directly into vapor under vacuum?