Question Detail

BIO 201 Objective Question
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Question

In real-time quantitative PCR (qPCR) assays, how is the cycle threshold (Ct value) related to initial target nucleic acid quantity?
Options
A Inversely proportional to the logarithm of initial nucleic acid concentration
Correct Answer
B Directly proportional to total sample protein content
C Uncorrelated to target copy number
D Linearly proportional without logarithmic scaling
Correct Answer

Option A is the correct answer.

Detailed Explanation

Lower Ct values reflect a lower number of PCR amplification cycles required to cross the detection threshold, indicating a higher starting concentration of target nucleic acid.

Hint

No hint available

Question Info

Course: BIO 201
Type: Objective
Difficulty: Not specified
Question 23 of 285
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