Question Detail

BIO 201 Objective Question
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Question

What is the primary role of a non-template control (NTC) in parallel qPCR diagnostic diagnostic runs?
Options
A To detect nucleic acid contamination or primer-dimer formation
Correct Answer
B To normalize fluorescent signal intensity across sample wells
C To construct the standard curve for target quantification
D To evaluate the enzymatic activity of reverse transcriptase
Correct Answer

Option A is the correct answer.

Detailed Explanation

NTC reaction mixtures contain all reagents except target DNA/RNA; fluorescence amplification in an NTC signals reagent contamination or non-specific primer dimerization.

Hint

No hint available

Question Info

Course: BIO 201
Type: Objective
Difficulty: Not specified
Question 24 of 285
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