Question Detail

BIO 201 Objective Question
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Question

In cell biology assays used within diagnostic workflows, what is the underlying mechanism of the Trypan Blue exclusion assay?
Options
A Intact cell membranes exclude the dye, while damaged membranes allow dye entry into viable-compromised cells
Correct Answer
B Metabolically active mitochondria reduce the dye into a purple formazan product
C The dye selectively intercalates into double-stranded DNA inside living cell nuclei
D Active efflux pumps extrude the dye exclusively from apoptotic cells
Correct Answer

Option A is the correct answer.

Detailed Explanation

Trypan blue is a membrane-impermeant dye; non-viable cells with damaged membranes take up the blue dye, while viable cells exclude it.

Hint

No hint available

Question Info

Course: BIO 201
Type: Objective
Difficulty: Not specified
Question 33 of 285
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