Question Detail

BIO 201 Objective Question
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Question

What is an internal amplification control (IAC) used for in diagnostic PCR assays?
Options
A To confirm the absence of PCR inhibitors in the reaction vessel
Correct Answer
B To quantify total human genomic DNA in non-clinical samples
C To induce Taq polymerase activation via heat denaturation
D To eliminate non-specific binding of primary antibodies
Correct Answer

Option A is the correct answer.

Detailed Explanation

An IAC is a non-target DNA sequence co-amplified in the same tube; failure to amplify the IAC signals sample inhibition, preventing false-negative interpretations.

Hint

No hint available

Question Info

Course: BIO 201
Type: Objective
Difficulty: Not specified
Question 35 of 285
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