Question Detail

BIO 201 Objective Question

Question

In flow cytometry diagnostic assays, what is the process of 'gating'?
Options
A Selecting a specific subpopulation of cells based on physical light scatter and fluorescence parameters for targeted analysis
Correct Answer
B Physically filtering large cell aggregates through a mesh screen before sample acquisition
C Adjusting voltage to prevent detector saturation during laser exposure
D Cleaving surface markers using trypsin prior to fluorescence labeling
Correct Answer

Option A is the correct answer.

Detailed Explanation

Gating isolates specific cell populations (e.g., lymphocytes via forward/side scatter) to quantify marker expression strictly within that subset.

Hint

No hint available

Question Info