Question Detail

BIO 201 Objective Question

Question

What is the primary function of fluorophore compensation in multi-color flow cytometry diagnostic panels?
Options
A Correcting for spectral overlap between different fluorescent emission channels
Correct Answer
B Increasing the physical velocity of single-cell fluidic droplets
C Enzymatically digesting cell surface receptors to reduce background signal
D Preventing photobleaching of cell nucleoli during laser exposure
Correct Answer

Option A is the correct answer.

Detailed Explanation

Compensation mathematically removes fluorescence emission spillover from one fluorophore out of secondary detector channels measuring other fluorophores.

Hint

No hint available

Question Info