Question Detail

BIO 201 Objective Question

Question

In parallel microplate diagnostic assays (e.g., ELISA), how is intra-assay variability primarily measured?
Options
A By calculating the coefficient of variation (%CV) across replicate wells within the same plate run.
Correct Answer
B By comparing average absorbance values across plates processed on different days.
C By subtracting negative control absorbance from positive control absorbance.
D By dividing total sample size by the number of serial dilutions.
Correct Answer

Option A is the correct answer.

Detailed Explanation

Intra-assay variability reflects precision across duplicate or triplicate wells processed concurrently within a single analytical run.

Hint

No hint available

Question Info